Batch Oligo Design (Cont.)
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There are four frequently used points for a typical mRNA sequence: the 5’ end, ATG (the initiation codon), Stop (the termination codon) and the 3’ end. Any of the four can be used as a reference point. Counting bases from any point towards the 5’ end is considered as minus, and plus if towards the 3’ end.
Setting forward oligo parameters:
Setting reverse oligo parameters:
Sample oligos designed by the program:
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