Oligo Design

Objective: Design oligos from a DNA sequence.

A successful DNA amplification by PCR is partly dependent upon the pair of oligos used. Optimized oligos can increase the yield of the amplified DNA and reduce the background caused by non-specific reactions. The Oligo Design function in GeneLooper™ 2.0 uses a reliable algorithm to ensure selection of the best pair of oligos from the defined regions in a DNA sequence.

Features:

1. Easy to setup: all-in-one design interface.
2. Diagram with ORF information, and sliding guidelines and pull-down menus for precisely defining regions for selecting oligos.
3. Dynamically detect the internal restriction sites to guide selection of enzyme sites for addition to the 5’ ends of the oligos.
4. One step addition of restriction site and protection bases.
5. Return the best pair or multiple pairs of oligos with better scores in a spreadsheet.

A sample of working interface: